Publications 2025

Lind S, Wright SC, Gvozdenovic E, Nilsson K, Granberg KL, Bouvier M, Johansson LC. Profiling of HCAR1 signalling reveals Gαi/o and Gαs activation without β-arrestin recruitment and the discovery of an allosteric agonist. Br J Pharmacol. 2025 Dec 23. doi: 10.1111/bph.70312.

Wijewardane A, Fernandez KX, Zhang A, Fischer C, Nagahawatta DP, Iturrioz X, Avet C, Heard BL, Llorens-Cortes C, Oudit GY, Bouvier M, Vederas JC. Screening apelin analogues and a small molecule agonist as effective cardiovascular therapeutics against reperfusion injury. RSC Med Chem. 2025 Dec 19. doi: 10.1039/d5md00985e.

Gray MW, Valach M, Sarrasin M, Le Sieur FA, Lukeš J, Burger G. The mitochondrial proteome of diplonemids: from conventional pathways to eccentric RNA editing and transcript processing. BMC Genomics. 2025 Dec 11;26(1):1099.

Robu M, Shah RG, van den Heuvel D, Coulombe Y, Bazin M, van der Woude M, Kragten A, Lans H, Pascal JM, Masson JY, Luijsterburg MS, Shah GM. PARP1 and PARylation facilitate transcription-coupled DNA repair by stabilizing the CSB-RNAPII complex. Nucleic Acids Res. 2025 Nov 26;53(22):gkaf1303. doi: 10.1093/nar/gkaf1303.

Salazar S, Delgadillo-Silva LF, Carapeto P, Kenfaoui MM, Dakessian K, Melhem R, Provencher-Girard A, Ostinelli G, Turgeon J, Kaci I, Migneault F, Huising MO, Hébert MJ, Chaker-Margot M, Rutter GA. Additive Effects of Dorzagliatin and Glucagon-Like Peptide-1 Receptor Agonism in a Novel Mouse Model of GCK-MODY and in Obese db/db Micedroui. Diabetes. 2025 Nov 6:db250520. doi: 10.2337/db25-0520.

Richard-Carpentier G, Béliveau F, Lacoste S, Khakipoor B, Lisi V, Vladovsky M, Marquis M, Spinella JF, Gendron P, Lemieux S, Lavallée VP, Sauvageau G, Hébert J. High IL1R1 expression predicts poor survival and benefit from stem cell transplant in intermediate-risk acute myeloid leukemia from the Leucegene cohort. Biomark Res. 2025 Oct 23;13(1):133.

Petraccione K, Omichinski JG, Kehn-Hall K. Immune Evasion by the NSs Protein of Rift Valley Fever Virus: A Viral Houdini Act. Viruses. 2025 Oct 21;17(10):1398.

Bouchard AY, Vivet AJICabana VC, Li C, Thibault P, Lussier MP, Mader S, Cappadocia L. A short SUMOylation tag modulates transcription factor activity. J Biol Chem. 2025 Oct 8:110807. doi: 10.1016/j.jbc.2025.110807.

Gencel M, Gagné-Leroux D, Serohijos AWR. Doblin: Inferring dominant clonal lineages from high-resolution DNA barcoding time series. Bioinformatics. 2025 Oct 6:btaf555. doi: 10.1093/bioinformatics/btaf555.

Faraway R, Zenklusen D, Plaschka C. Mechanisms of Messenger RNA Packaging and Export. Annu Rev Cell Dev Biol. 2025 Oct;41(1):479-504.

Higazy-Mreih S, Avital-Shacham M, LeGouill C, Bouvier M, Kosloff M. Gαs-specific structural elements attenuate interactions with regulator of G protein signaling (RGS) proteins. FEBS J. 2025 Oct;292(19):5244-5254. doi: 10.1111/febs.70149.

Adams JJ, Blazer LL, Chung J, Karimi M, Davidson T, Bruce HA, Singer AU, Yang N, Cardarelli L, Pot I, Colombo L, Huang LJ, Ma Y, Michnick SW, Moe OW, Sidhu SS. An asymmetric tetrabody is a potent and efficacious agonist of the erythropoietin receptor in vitro and in vivo. Protein Sci. 2025 Oct;34(10):e70292.

Lemire L, Reyes SJ, Durocher Y, Voyer R, Henry O, Pham PL. Scale-up of a monoclonal antibody CHO fed-batch production in stirred tank bioreactors: Effect of hydrodynamic conditions and feeding regimen. Biotechnol Prog. 2025 Sep 29:e70073.

Langelier MF, Pascal JM. PARP enzyme synthesis of protein-free poly(ADP-ribose): Implications for DNA damage signaling and repairPARP enzyme synthesis of protein-free poly(ADP-ribose): Implications for DNA damage signaling and repair – ScienceDirect. DNA Repair (Amst). 2025 Sep 27;154:103898.

Sohail I, Laurin SA, Kleinau G, Chunilal V, Morton A, Brenlla A, Kagiali ZCU, Blouin MJ, Tello JA, Beck-Sickinger AG, Lohse MJ, Scheerer P, Bouvier M, McCormick P, Annibale P, Biebermann H. MRAP2 modifies the signaling and oligomerization state of the melanocortin-4 receptor. Nat Commun. 2025 Sep 25;16(1):8324.

Nahlé S, Javed A, Joumier L, Kherdjemil Y, Sitolle J, Khetchoumian K, Parekh Y, Krezel W, Malleshaiah M, Le Grand F, Cayouette M, Côté JF. Differentially expressed fusogens specify myocyte states to drive myogenesis.  Development. 2025 Sep 17:dev.204771.

Rostami-Afshari B, Elremaly W, McGregor NR, Huang KJK, Armstrong CW, Franco A, Godbout C, Elbakry M, Abdelli R, Moreau A. Circulating Levels of SMPDL3B Define Metabolic Endophenotypes and Subclinical Kidney Alterations in Myalgic Encephalomyelitis. Int J Mol Sci. 2025 Sep 12;26(18):8882.

Fineberg D, Moreau A, Schneider-Futschik EK, Armstrong CW. A Perspective on the Role of Metformin in Treating Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and Long COVID. ACS Pharmacol Transl Sci. 2025 Sep 11;8(10):3411-3431.

Fuentes Valenzuela L, Francois P, Skotheim JM. The Product neutrality function defining genetic interactions emerges from mechanistic models of cell growth. Elife. 2025 Sep 2;14:RP105265.

Moezzi A, Ushenkina A, Widgren A, Bergquist J, Li P, Xiao W, Rostami-Afshari B, Leveau C, Elremaly W, Caraus I, Franco A, Godbout C, Nepotchatykh O, Moreau A. Haptoglobin phenotypes and structural variants associate with post-exertional malaise and cognitive dysfunction in myalgic encephalomyelitis. J Transl Med. 2025 Aug 28;23(1):970.

Stuible M, Alpuche-Lazcano SP, Gervais C, Ouimet M, Lippens J, Pagé M, Morasse A, Moraitis AN, Durocher Y. Endogenous Retrovirus-Like Particle-Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell-Surface Expression of Retroviral Envelope Protein. Biotechnol Bioeng. 2025 Aug 22. doi: 10.1002/bit.70043.

Moreno-Salinas AL, Mancini A, Aouad S, Kandasamy H, Morissette S, Maiga A, Bouvier M, Leduc R, Sabbagh L. Decoding ADGRE5: How Proteolytic Cleavage and Mechanical Forces Unleash Cellular Signals. Cells. 2025 Aug 19;14(16):1284.

Gaonac’h-Lovejoy V, Mattick JS, Sauvageau M, Smith MA. ECSFinder: optimized prediction of evolutionarily conserved RNA secondary structures from genome sequences. Nucleic Acids Res. 2025 Aug 11;53(15):gkaf780.

Azimi G, Elremaly W, Elbakry M, Franco A, Godbout C, Moreau A. Circulating FGF-21 as a Disease-Modifying Factor Associated with Distinct Symptoms and Cognitive Profiles in Myalgic Encephalomyelitis and Fibromyalgia. Int J Mol Sci. 2025 Aug 8;26(16):7670.

O’Brien SL, Tripp E, Barki N, Blondel-Tepaz E, Smith G, Boufersaoui A, Roberts J, Pike JA, Correia J, Miljus T, Bouvier M, Tennant DA, Hudson BD, Gerhart-Hines Z, Milligan G, Schwartz TW, Calebiro D. Intracrine FFA4 signaling controls lipolysis at lipid droplets. Nat Chem Biol. 2025 Aug 5. doi: 10.1038/s41589-025-01982-5.

Usama M, Deng Y, Chen Y, Milland T, Malleshaiah M, Aghazadeh Y. Navigating challenges in human pluripotent stem cell-derived islet therapy for type 1 diabetes. Front Immunol. 2025 Aug 4;16:1625439.

Baniahmad SF, Burlacu A, Delafosse L, Acchione M, Simmons M, Ling B, Iqbal U, Moreno MJ, De Crescenzo G, Durocher Y. Use of Coiled-Coil Affinity Peptides to Manufacture Antibody Conjugates. Bioconjug Chem. 2025 Jul 29. doi: 10.1021/acs.bioconjchem.5c00178.

Kamada R, Sakaguchi S, Kanno M, Ozawa T, Nakagawa N, Omichinski JG, Sakaguchi K. Dominant-Negative Effects of p53 R337 Variants in Li-Fraumeni Syndrome: Impact on Tetramer Formation and Transcriptional Activity. Chembiochem. 2025 Jul 24:e2500330. doi: 10.1002/cbic.202500330.

Mukhopadhyay J, Wai A, Lang BF, Hausner G. Characterization of the mitochondrial genomes for Ophiostomaips and related taxa from various geographic origins and related species: large intron-rich genomes and complex intron arrangements. IMA Fungus. 2025 Jul 22;16:e159349.

Denis CM, Mochulska V, Dagher M, Verbavatz V, Bourassa FXP, Altan-Bonnet G, François P. Dynamical model and geometric insights in the discontinuity theory of immunity. bioRxiv [Preprint]. 2025 Jul 18:2025.07.16.663927.

Gencel M, Cofino GM, Hui C, Sahaf Z, Gauthier L, Matta C, Gagné-Leroux D, Tsang DKL, Philpott DP, Ramathan S, Menendez A, Bershtein S, Serohijos AWR. Quantifying the intra- and inter-species community interactions in microbiomes by dynamic covariance mapping. Nat Commun. 2025 Jul 9;16(1):6314.

Rostami-Afshari B, Elremaly W, Franco A, Elbakry M, Akoume MY, Boufaied I, Moezzi A, Leveau C, Rompré P, Godbout C, Mella O, Fluge Ø, Moreau A. SMPDL3B a novel biomarker and therapeutic target in myalgic encephalomyelitis. J Transl Med. 2025 Jul 7;23(1):748.

Metayer I, Forest-Nault C, Guimond J, Joubert S, Henry O, Durocher Y, De Crescenzo G, Gaudreault J. A Surface Plasmon Resonance-Based Integrated Assay for Quantification and Glycosylation Characterization of Monoclonal Antibodies in Crude Heterogeneous Samples. Biotechnol Bioeng. 2025 Jul 5. doi: 10.1002/bit.70016.

Ahmed O, Masclef L, Iannantuono N, Gagnon J, Gushul-Leclaire M, Boulay K, Estavoyer B, Echbicheb M, Ali Boubacar K, Poy M, Boubekeur A, Menggad S, Schcolnik-Cabrera A, Balsalobre A, Bonneil E, Thibault P, Hulea L, Mallette FA, Drouin J, Tanaka Y, Affar EB. O-GlcNAcylation of FOXK1 co-opts BAP1 to orchestrate the E2F pathway and promotes oncogenesis. Nat Commun. 2025 Jul 1;16(1):5959.

Doucet R, Elseoudi A, Rostami-Afshari B, Elbakry M, Taheri M, Pellicelli M, Picard C, Lavoie JF, Wang DS, Lavigne P, Gorman KF, Elremaly W, Moreau A. UBC9-Mediated SUMO Pathway Drives Prohibitin-1 Nuclear Accumulation and PITX1 Repression in Primary Osteoarthritis. Int J Mol Sci. 2025 Jun 29;26(13):6281.

Rodriguez MM, Chatterjee D, Guerry J, Patenaude AM, Cohen CCH, Bois T, Larouche A, Ferreira SR, Bertomeu T, Chatr-Aryamontri A, Zhang L, Mader S, Nislow C, St-Jean G, Guindon Y, Zahn A, Di Noia JM. CDADC1 is a vertebrate-specific dCTP deaminase that metabolizes gemcitabine and decitabine to prevent cellular toxicity. Proc Natl Acad Sci U S A. 2025 Jun 17;122(24):e2424409122.

Morales-Pastor A, Miljuš T, Dieguez-Eceolaza M, Stępniewski TM, Ledesma-Martin V, Heydenreich FM, Flock T, Plouffe B, Le Gouill C, Duchaine J, Sykes DA, Nicholson C, Koers EJ, Guba W, Rufer AC, Grether U, Bouvier M, Veprintsev DB, Selent J. Multiple intramolecular triggers converge to preferential G protein coupling in the CB2R. Nat Commun. 2025 Jun 11;16(1):5265.

Reyes SJ, Voyer R, Durocher Y, Henry O, Pham PL. A recurrent neural network for soft sensor development using CHO stable pools in fed-batch process for SARS-CoV-2 spike protein production as a vaccine antigen. Biotechnol Prog. 2025 Jun 2:e70046. doi: 10.1002/btpr.70046.

Bourassa FXP, François P, Reddy G, Vergassola M. Manifold learning for olfactory habituation to strongly fluctuating backgrounds. bioRxiv [Preprint]. 2025 May 30:2025.05.26.656161.

Mignaqui AC, Ferella A, Sánchez C, Stuible M, Scian R, Filippi J, Cardillo SB, Durocher Y, Wigdorovitz A. Optimized Production of Virus-like Particles in a High-CHO-Cell-Density Transient Gene Expression System for Foot-and-Mouth Disease Vaccine Development. Vaccines (Basel). 2025 May 29;13(6):581.

Higazy-Mreih S, Avital-Shacham M, LeGouill C, Bouvier M, Kosloff M. Gαs-specific structural elements attenuate interactions with regulator of G protein signaling (RGS) proteins. FEBS J. 2025 May 28. doi: 10.1111/febs.70149.

Gemeinhardt TM, Regy RM, Phan TM, Pal N, Sharma J, Senkovich O, Mendiola AJ, Ledterman HJ, Henrickson A, Lopes D, Kapoor U, Bihani A, Sihou D, Kim YC, Jeruzalmi D, Demeler B, Kim CA, Mittal J, Francis NJ. A disordered linker in the Polycomb protein Polyhomeotic tunes phase separation and oligomerization. Mol Cell. 2025 May 23:S1097-2765(25)00413-7.

Frigon L, Pascal JM. Crystal structure and mutagenesis of a nucleic acid-binding BRCT domain in human PARP4. J Biol Chem. 2025 May 22:110277. doi: 10.1016/j.jbc.2025.110277.

Apavaloaei A, Zhao Q, Hesnard L, Cahuzac M, Durette C, Larouche JD, Hardy MP, Vincent K, Brochu S, Laverdure JP, Lanoix J, Courcelles M, Gendron P, Lajoie M, Ruiz Cuevas MV, Kina E, Perrault J, Humeau J, Ehx G, Lemieux S, Watson IR, Speiser DE, Bassani-Sternberg M, Thibault P, Perreault C. Tumor antigens preferentially derive from unmutated genomic sequences in melanoma and non-small cell lung cancer. Nat Cancer. 2025 May 22. doi: 10.1038/s43018-025-00979-2.

Lopes-Paciencia S, Ferbeyre G. Increased chromatin accessibility underpins senescence. FEBS J. 2025 May 19. doi: 10.1111/febs.70136.

Laflamme R, Lisi V, Hébert J, Sauvageau G, Lemieux S, Lavallée VP, Lettre G. Replication of a GWAS signal near HLA-DQA2 with AML using a disease-only cohort and external population-based controls. Blood Neoplasia. 2025 May 19;2(3):100118.

Harris J, Mayran A, Gouhier A, Gauthier Y, Sleiman NH, Merabet S, Dukatz M, Bashtrykov P, Jeltsch A, Djambazian H, Chen SH, Balsalobre A, Drouin J. Dual DNA demethylation mechanisms implement epigenetic memory driven by the pioneer factor PAX7. Sci Adv. 2025 May 16;11(20):eadu6632.

Fortin A, Huot S, Caron E, Laflamme C, Pagliuzza A, Chomont N, Gilbert C, Zhang B, Kwong PD, Pouliot M, Grandvaux N. No Evidence of Direct Activation of Human Neutrophil Responses by Multivalent Prefusion Trimeric SARS-CoV-2 Spike Protein ex vivo. bioRxiv [Preprint]. 2025 May 8:2025.05.08.652856.

Kondo T, Bourassa FXP, Achar S, DuSold J, Céspedes PF, Ando M, Dwivedi A, Moraly J, Chien C, Majdoul S, Kenet AL, Wahlsten M, Kvalvaag A, Jenkins E, Kim SP, Ade CM, Yu Z, Gaud G, Davila M, Love P, Yang JC, Dustin ML, Altan-Bonnet G, François P, Taylor N. Engineering TCR-controlled fuzzy logic into CAR T cells enhances therapeutic specificity. Cell. 2025 May 1;188(9):2372-2389.e35.

Padilla JA, Barutcu S, Boulais J, da Silva JC, Chen Y, Syed E, Kwon E, Lécuyer E. Inhibition of acid or neutral sphingomyelinases differentially impacts RNA and protein cargo sorting to extracellular vesicles. iScience. 2025 Apr 15;28(5):112440.

Métois A, Bordeleau ME, Theret L, Hajmirza A, Moujaber O, Spinella JF, Chagraoui J, Mayotte N, Boivin I, Audemard É, Aubert L, Lisi V, Khakipoor B, Farah A, Bonneil É, Robert A, Lippens J, Moraitis A, Béliveau F, Feghaly A, Boucher G, Marcotte R, Gendron P, Thibault P, Lemieux S, Richard-Carpentier G, Lavallée VP, Hébert J, Roux PP, Sauvageau G. IL1RAP is an immunotherapeutic target for normal karyotype triple-mutated acute myeloid leukemia. Biomark Res. 2025 Apr 14;13(1):61.

Renner TM, Stuible M, Rossotti MA, Rohani N, Cepero-Donates Y, Sauvageau J, Deschatelets L, Dudani R, Harrison BA, Baardsnes J, Koyuturk I, St Michael F, Hill JJ, Hemraz UD, Lenferink AEG, Tanha J, Fernandes B, Roldao A, McCluskie MJ, Akache B, Durocher Y. Modifying the glycosylation profile of SARS-CoV-2 spike-based subunit vaccines alters focusing of the humoral immune response in a mouse model. Commun Med (Lond). 2025 Apr 11;5(1):111.

Kaya F, Bewicke-Copley F, Miettinen JJ, Casado P, Leddy E, Deniz Ö, Lavallée VP, Philippe C, Zheng J, Grebien F, Khan N, Krizsán S, Saad J, Nolin-Lapalme A, Hébert J, Lemieux S, Audemard E, Matthews J, Grantham M, Di Bella D, Wennerberg K, Parsons A, Gribben J, Cavenagh JD, Freeman SD, Bödör C, Sauvageau G, Wang J, Llamas-Sillero P, Cazier JB, Taussig DC, Bonnet D, Cutillas PR, Heckman CA, Fitzgibbon J, Rouault-Pierre K, Rio-Machin A. DEK::NUP214 acts as an XPO1-dependent transcriptional activator of essential leukemia genes. Leukemia. 2025 Apr 9. doi: 10.1038/s41375-025-02593-8.

Lemire L, Reyes SJ, Durocher Y, Voyer R, Henry O, Pham PL. N-1 semi-continuous transient perfusion in shake flask for ultra-high density seeding of CHO cell cultures in benchtop bioreactors. Biotechnol Prog. 2025 Apr 3:e70029.

Thimmappa BC, Sarrasin M, Lang BF, Burger G. The draft genome sequence of Diaporthe vaccinii, isolated from diseased cranberries. Microbiol Resour Announc. 2025 Apr 2:e0046624.

Carroll M, Fox HB, Tran A, Chellappan G, Rojas LV, Karengil G, Karandish F, Langston JW, Fall BM, Whalen MM, McCluskie MJ, Durocher Y, Datta A, Kapre SV, Olave IA. SARS-CoV-2 conjugate vaccine elicits robust immune responses that can protect against evolving variants. Vaccine. 2025 Mar 6;54:126988.

Francis NJ. A chromatin mesh model for compaction of chromatin by PRC1 in condensates. Nat Struct Mol Biol. 2025 Mar;32(3):411-413.

Ferbeyre G. A one-two punch against cancer: combining CDK4 inhibitors with lysosomotropic agents. EMBO J. 2025 Feb 24. doi: 10.1038/s44318-025-00396-2.

Li J, Wang X, Jordana L, Bonneil É, Ginestet V, Ahmed M, Bourouh M, Pascariu CM, Schmeing TM, Thibault P, Archambault V. Mechanisms of PP2A-Ankle2 dependent nuclear reassembly after mitosis. Elife. 2025 Feb 18;13:RP104233.

Teixeira LB, Blouin MJ, Le Gouill C, Picard LP, Costa-Neto CM, Bouvier M, Parreiras-E-Silva LT. Sustained Gαs signaling mediated by vasopressin type 2 receptors is ligand dependent but endocytosis and β-arrestin independent. Sci Signal. 2025 Feb 18;18(874):eadf6206.

Khatami N, Caraus I, Rahaman M, Nepotchatykh E, Elbakry M, Elremaly W, Franco A, Beauséjour M, Laberge AM, Parent S, Labelle H, Aubin CÉ, Lachaine J, Moreau A. Genome-wide profiling of circulating microRNAs in adolescent idiopathic scoliosis and their relation to spinal deformity severity, and disease pathophysiology. Sci Rep. 2025 Feb 12;15(1):5305.

Panichnantakul P, Oeffinger M. Protocol for the purification and analysis of nuclear UFMylated proteins. STAR Protoc. 2025 Feb 10;6(1):103634.

Reyes SJ, Pham PL, Durocher Y, Henry O. CHO stable pool fed-batch process development of SARS-CoV-2 spike protein production: Impact of aeration conditions and feeding strategies. Biotechnol Prog. 2025 Jan-Feb;41(1):e3507.

Elbakry M, Khatami N, Akoume MY, Julien C, Bouhanik S, Franco A, Caraus I, Elremaly W, Moreau A. Loss of Tyrosine Phosphatase Mu Promotes Scoliosis Progression Through Osteopontin-α5β1 Integrin Signaling and PIPK1γ90 Activity. Int J Mol Sci. 2025 Jan 26;26(3):1042.

Smith-Pillet ES, Billur R, Langelier MF, Talele TT, Pascal JM, Black BE. A PARP2 active site helix melts to permit DNA damage-induced enzymatic activation. Mol Cell. 2025 Jan 24:S1097-2765(25)00004-8.

Nieman LK, Castinetti F, Newell-Price J, Valassi E, Drouin J, Takahashi Y, Lacroix A. Cushing syndrome. Nat Rev Dis Primers. 2025 Jan 23;11(1):4.

Reyes SJ, Lemire L, Durocher Y, Voyer R, Henry O, Pham PL. Investigating the metabolic load of monoclonal antibody production conveyed to an inducible CHO cell line using a Transfer-Rate Online Monitoring system. J Biotechnol. 2025 Jan 17:S0168-1656(25)00008-2.

Cheng SJ, Gafaar T, Kuttiyatveetil JRA, Sverzhinsky A, Chen C, Xu M, Lilley A, Pascal JM, Leung AKL. Regulation of stress granule maturation and dynamics by poly(ADP-ribose) interaction with PARP13. Nat Commun. 2025 Jan 13;16(1):621.

Hashimoto-Roth E, Forget D, Gaspar VP, Bennett SAL, Gauthier MS, Coulombe B, Lavallée-Adam M. MAGPIE: A Machine Learning Approach to Decipher Protein-Protein Interactions in Human Plasma. J Proteome Res. 2025 Jan 7.

Bonneil É, Larouche M, Emond-Fraser V, Kubiniok P, Pascariu CM, Thibault P, Archambault V. Studying Mitotic Phosphorylation in Drosophila. Methods Mol Biol. 2025;2874:167-182.

Padilla JA, Barutcu S, Deschamps-Francoeur G, Lécuyer E. Exploring Extracellular Vesicle Transcriptomic Diversity Through Long-Read Nanopore Sequencing. Methods Mol Biol. 2025;2880:227-241.