BIOMOLECULAR NMR

Applications

Analysis of small molecules, peptides and biomolecules of small molecular weight. Biomolecule (protein, RNA, DNA) – small molecule titrations. Rapid assessment of the quality of a biomolecular sample in order to initiate biophysical studies.

NMR tubes : 3 or 5 mm in diameter (standard or Shigemi)

Temperature : 0 – 80 ˚C

Magnet and console

Shielded, superconducting Oxford magnet of 500 MHz (11.7 Tesla) coupled to a Bruker AVANCE NEO console.

Probes available

Normally equipped with a 5 mm TXI probe (inverse triple resonance) with automatic tuning and matching and Z-gradient capability. This probe is designed for ¹H observation with decoupling capacity of ¹³C, ¹⁵N and ²D.

A second probe is available if needed: a 5 mm QXI probe (inverse quadruple resonance) with automatic tuning and matching and Z-gradient capability. This probe is designed for ¹H observation with decoupling capacity of ¹³C, ¹⁵N and ³¹P in the same experiment. Can also be used for ¹³C, ¹⁵N and ³¹P observed experiments, albeit not optimized for that purpose.

Logiciels

• TopSpin 4.0.3 (Linux CentOS)
• Complete Molecular Confidence suite (CMC-assist and CMC-se)
• Non-Uniform Sampling (NUS)
• Protein Dynamics Center

Technical support

Normand Cyr, Structural biology platform manager normand.cyr@umontreal.ca

Applications

Improved sensitivity with cryo-probe usage. Ideal for multi-dimensional, triple resonance experiments typically used in structural and dynamics studies of proteins and nucleic acids.

NMR tubes : 3 or 5 mm in diameter (standard or Shigemi) and Bruker shaped tubes (with SPU)

Temperature : 0 – 80 ˚C

Magnet and console

Superconducting Oxford magnet of 600 MHz (14.1 Tesla) coupled to a Bruker AVANCE NEO console.

Probes available

Normally equipped with a TCI 5 mm cryo-probe (inverse triple resonance) with automatic tuning and matching, Z-gradient capability and sample positioning unit (SPU). This probe is designed for high sensitivity ¹H observation (with ¹³C, ¹⁵N and ²D decoupling) as well as ¹³C observation (with ¹H, ¹⁵N and ²D decoupling). The pre-amplifier for the lock signal (²D) is also cryo-cooled thus enabling the usage of lower percentages of deuterated solvent.

A second probe is also available on demand: a 5 mm TXI probe (inverse triple resonance) with automatic tuning and matching and Z-gradient capability. This probe is designed for ¹H observation with decoupling capacity of ¹³C, ¹⁵N and ²D.

Softwares

• TopSpin 4.0.3 (Linux CentOS)
• Complete Molecular Confidence suite (CMC-assist and CMC-se)
• Non-Uniform Sampling (NUS)
• Protein Dynamics Center

Technical support

Normand Cyr, Structural biology platform manager normand.cyr@umontreal.ca

Applications

Great resolution and sensitivity. Ideal for multi-dimensional, triple resonance experiments typically used in structural and dynamics studies of proteins and nucleic acids.

NMR tubes : 3 or 5 mm in diameter (standard or Shigemi)

Temperature : 0 – 80 ˚C

Magnet and console

Shielded, superconducting Bruker Ascend magnet of 700 MHz (16.4 Tesla) coupled to a Bruker AVANCE NEO console.

Probes

Normally equipped with a 5 mm TXI probe (inverse triple resonance) with automatic tuning and matching and Z-gradient capability. This probe is designed for ¹H observation with decoupling capacity ¹³C, ¹⁵N and ²D.

A second probe is also available on demand: a BBI 5 mm probe (inverse broadband) with automatic tuning and matching and Z-gradient capability. This probe enables the observation of ¹H with decoupling capacity for a single nucleus between ³¹P and ¹⁰⁹Ag depending on the tuning. Useful for HSQC, HMQC and HMBC experiments. Can be used for the observation of ¹³C, ¹⁵N or ³¹P observed experiments, albeit not optimized for that purpose.

Softwares

• TopSpin 4.0.3 (Linux CentOS)
• Complete Molecular Confidence suite (CMC-assist & CMC-se)
• Non-Uniform Sampling (NUS)
• Protein Dynamics Center

Technical support

Normand Cyr, Structural biology platform manager normand.cyr@umontreal.ca